General Cell Based Assays
Eurofins Discoverx PathHunter eXpress HRH4 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0209E3CP0L)
The PathHunter HRH4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. histamine) based activation of HRH4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HRH4 and EA-tagged β-Arrestin-2. Activation of HRH4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical SIRT1 FRETBased Screening Ass
Human SIRT1 is the homolog of yeast Sir2 and has been shown to regulate the activity of the p53 tumor suppressor and inhibit apoptosis. Cayman’s SIRT1 FRET-based Screening Assay provides a convenient fluorescence-based method for screening SIRT1 inhibitors or activators. The procedure requires only two EAsy steps, both performed in the same microplate. In the first step, the substrate, which is coupled to the fluorophore and quencher, is incubated with human recombinant SIRT1 along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step results in the separation of the quencher and fluorophore. The resulting fluorescence is analyzed using an excitation wavelength between 335-345 nm and emission wavelength between 440-465 nm.
Abcam Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit
Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
ABclonal Technology LDH-B
This gene encodes the B subunit of lactate dehydrogenase enzyme which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD in a post-glycolysis process Alternatively spliced transcript variants have been found for this gene Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism and that this isoform is localized in the peroxisomes Mutations in this gene are associated with lactate dehydrogenase B deficiency Pseudogenes have been identified on chromosomes X 5 and 13
Eurofins Discoverx PathHunter eXpress GLP1R CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0300E2CP0M)
The PathHunter GLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP1R and EA-tagged β-Arrestin-2. Activation of GLP1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-YAP (Ser127) Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Phospho-YAP (Ser127) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Testosterone Detection Kit, 10,000 Assay Points
HTRF Testosterone Detection Kit, 10,000 Assay Points
Meso Scale Discovery U-PLEX Human IL-1 ASY
The Meso Scale Discovery U-PLEX Human IL-1alpha Assay Kit is a multiplex immunoassay that measures IL-1alpha in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements The assay can quantify IL-1alpha concentrations between 0 06 pg/mL to 10 000 pg/mL It includes a 96-well plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent labels calibrators and an MSD read buffer The total assay time is 3 5 hours
Cayman Chemical GGT Substrate 1ea
GGT substrate has been tested and formulated to work exclusively with Cayman’s γ-Glutamyl Transferase Activity Assay Kit (Item No. 702210).